Abstract The discovery of secondary metabolites from marine microorganisms is beset by numerous challenges including difficulties cultivating and subsequently eliciting expression of biosynthetic genes from marine microbes in the laboratory. In this paper. we describe a method of culturing three species from the marine bacterial genus Pseudoalteromonas using cotton scaffold supplement... https://www.spidertattooz.com/CND-Shellac-Be-Demure-0-5oz-p13568/