Abstract RNA-binding proteins (RBPs) modulate all aspects of RNA metabolism. but a comprehensive picture of RBP expression across tissues is lacking. Here. we describe our development of the method we call HARD-AP that robustly retrieves RBPs and tightly associated RNA regulatory complexes from cultured cells and fresh tissues. https://www.roneverhart.com/Biermann-Marzipan-Fruit-Candy-Gift-Boxes-12-Piece-Case/