With the aim of developing bioprocesses for waste valorization and a reduced water footprint. we optimized a two-step fermentation process that employs the oleaginous yeast Cutaneotrichosporon oleaginosus for the production of oil from liquid cheese whey permeate. For the first step. the addition of urea as a cost-effective nitrogen source allowed an increase in yeast biomass production. https://www.alarecre.com/